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                                                                        CD Smolke, Curriculum Vitae


Contact Information
Christina D. Smolke
Assistant Professor of Chemical Engineering
Division of Chemistry and Chemical Engineering
1200 E. California Blvd., MC 210-41
California Institute of Technology
Pasadena, CA 91125
P: (626) 395-2460
F: (626) 568-8743
smolke@cheme.caltech.edu
http://cheme.che.caltech.edu/groups/cds/
Education
Postdoctorate, Molecular and Cell Biology, 2001 ­ 2003
University of California at Berkeley, Berkeley, CA
Topic: Role of Export in Nonsense-Mediated Decay in Saccharomyces cerevisiae
Mentor: Karsten Weis
Ph.D., Chemical Engineering, 2001
University of California at Berkeley, Berkeley, CA
Thesis Topic: Engineering the Stabilities of Polycistronic mRNA
Advisor: Jay D. Keasling
B.S., Chemical Engineering with Emphasis in Biology, 1997
University of Southern California, Los Angeles, CA
Professional Experience
2003-present   Assistant Professor (Dept. Chemical Engineering, Caltech)
2001-2003      NIH Postdoctoral Research Fellow (Dept. Molecular and Cell Biology, UCB)
1998-2000      Graduate Student Instructor (Dept. Chemical Engineering, UCB)
1997-2001      Graduate Research Assistant (Dept. Chemical Engineering, UCB)
1997           Process Engineer (Mobil Oil, Torrance, CA)
1996           Undergraduate Research Assistant (Dept. Chemical Engineering, UCLA)
1995           Facilities Engineer (Mobil Oil, Bakersfield, CA)
1994-1997      Laboratory Instructor (Information Technology Dept., USC)
1993-1997      Undergraduate Research Assistant (Dept. Chemical Engineering, USC)
Teaching Experience
2004-present   Instructor, graduate thermodynamics course and undergraduate biomolecular engineering
               laboratory (Caltech)
2000-2003      University GSI Teaching and Orientation Workshop Leader (UCB)
1998-2000      Graduate Student Instructor, graduate engineering mathematics course and undergraduate
               separations course (Dept. Chemical Engineering, UCB)
1994-1997      Laboratory Instructor (Information Technology Programming Dept., USC)
Membership / Services
2006-present   Member, Institute of Biological Engineers
2006           Session Chair, "Synthetic Systems Biology", American Institute of Chemical Engineers
               Annual Meeting
2006           Session Chair, "Microbial Metabolic Engineering", 232nd American Chemical Society
               National Meeting
2006           Session Chair, "Molecular Tools for the Metabolic Engineering", Society of Industrial
               Microbiology Annual Meeting


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                                                                         CD Smolke, Curriculum Vitae


2006           Technical Chair, "Synthetic Biology", Computational Systems Bioinformatics
               Conference
2005-present   Scientific Advisory Board, Codon Devices
2005-present   Scientific Chair, Synthetic Biology, Life Sciences Society
2005-present   Member, Biodefense Council
2005-present   Member, Kavli Nanoscience Institute (Caltech)
2005           Session Chair, "Synthetic Systems Biology", American Institute of Chemical Engineers
               Annual Meeting
2004-present   Steering Committee, Synthetic Biology Conference
2004           Co-organizer/Mentor, NSF-sponsored multi-school "Synthetic Biology Competition"
               (Caltech, MIT, Princeton, Boston University, UT Austin)
2004-present   Member, American Chemical Society
2004-present   Member, RNA Society
2004-present   Member, Center for Biological Circuit Design (Caltech)
2003           Participant, DARPA-sponsored ISAT study group on "Synthetic Biology"
2001-present   Ad Hoc Reviewer, Nature Chemical Biology, Bioinformatics, Biotechnological Progress,
               Biotechnology and Bioengineering, and Applied Biochemistry and Biotechnology
2000           Co-organizer/Mentor, NSF-sponsored MarBEC summer undergraduate research program
               (UCB)
1997-present   Member, American Institute of Chemical Engineers
Selected Honors
2005           Beckman Young Investigator Award
2004           TR100, Technology Review's Top 100 Young Innovators of the World
2001-2003      National Institute of Health Postdoctoral Fellowship
2001           Graduated Summa Cum Laude
2000-2001      UC Dissertation Year Fellowship
2000-2001      Shell Doctoral Fellowship
1999-2000      Chemical Engineering Departmental Citation for Outstanding Teaching
1998           DOW Prize for Excellence in Teaching
1998-1999      University Award for Outstanding Graduate Student Instructor
1997-2000      National Science Foundation Fellowship
1997           Tau Beta Pi Fellowship (Declined)
1997           Eugene Cota-Robles Fellowship (Declined)
1997           Graduated Summa Cum Laude
1997           Salutatorian
1997           Emma Josephine Bradely Bovard Student Award
1997           School of Engineering's Outstanding Service Award
1997           School of Engineering's Outstanding Student Award
1996           Golden Key Peat Marwick Junior Scholarship
1996           Unocal Scholarship
1996           Excellence Award in Biochemistry
1995           Southern California Section AIChE Outstanding Junior Award
1994           Outstanding Achievement Award in General Chemistry
1993-1997      Trustee Scholar Award
1993-1997      Merit Research Award
Patents
2005   Filed "Engineered yeast strains for the linear, tunable response of protein expression for a GAL
       promoter in response to galactose concentrations"
2005   Filed "Ampliswitch technology for an amplification-based, digital sensor device"



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                                                                           CD Smolke, Curriculum Vitae


2004    Filed "Ligand controlled RNA-based regulators of gene expression"
2004    Filed "Temperature controlled RNA-based regulators of gene expression"
Peer-Reviewed Publications
1. Win MN, Klein JS, Smolke CD. 2006. RNA aptamers to the benzylisoquinoline alkaloid codeine and
   the rapid characterization of their binding properties using surface plasmon resonance. In submission.
2. Pfleger BF, Pitera DJ, Smolke CD, Keasling JD. 2006. Combinatorial engineering of intergenic
   regions to tune expression of multiple genes in operons. In submission.
3. Hawkins KH, Smolke CD. 2006. The regulatory roles of the galactose permease and kinase in the
   induction response of the GAL network in Saccharomyces cerevisiae. In press. Journal of Biological
   Chemistry.
4. Bayer TS, Smolke CD. 2005. Programmable, ligand-controlled riboregulators of eukaryotic gene
   expression. Nat. Biotech. 23:337-343. News and Views: Plug and play with RNA. 306-307.
5. Smolke CD, Keasling JD. 2002. Effect of gene location, mRNA secondary structures, and RNase
   sites on expression of two genes in an engineered operon. Biotech. Bioeng. 80: 762-776.
6. Smolke CD, Keasling JD. 2002. Effect of copy number and mRNA processing and stabilization on
   transcript and protein levels from an engineered dual-gene operon. Biotech. Bioeng. 78: 412-424.
7. Smolke CD, Khlebnikov A, Keasling JD. 2001. Effects of transcription induction homogeneity and
   transcript stability on expression of two genes in a constructed operon. Appl. Micro. Biotech. 57: 689-
   696.
8. Smolke CD, Martin VJJ, Keasling JD. 2001. Controlling the metabolic flux through the carotenoid
   pathway using directed mRNA processing and stabilization. Met. Eng. 3: 313-321.
9. Smolke CD, Carrier TA, Keasling JD. 2000. Coordinated, differential expression of two genes
   through directed mRNA cleavage and stabilization by secondary structures. Appl. Environ. Microbiol.
   66: 5399-5405.
Non-Peer-Reviewed Publications
1. Smolke CD, editor. 2007. Metabolic Engineering Protocols, Vol 1. & 2. In Walker J, series ed.,
   Methods in Molecular Biology. Totowa: Humana Press. In preparation.
2. Smolke CD, editor. 2006. Handbook for Metabolic Pathway Engineering. San Diego: CRC Press. In
   preparation.
3. Bayer TS, Smolke CD. 2006. Design of nucleic acid switches. Nature Protocols. In preparation.
4. Bayer TS, Smolke CD. 2006. Nucleic acid switches as cellular biosensors. Biotechnology and Genetic
   Engineering Reviews, Vol. 24. In preparation.
5. Smolke CD, Martin VJJ, Keasling JD. 2004. Tools for metabolic engineering in Escherichia coli. In:
   Baneyx F., editor. Protein Expression Technologies: Current Status and Future Trends. U.K.: Horizon
   Scientific Press.
6. Martin VJJ, Smolke CD, Keasling JD. 2002. Redesigning cells for the production of complex organic
   molecules. ASM News 68: 336-343.
Selected Invited Presentations
Smolke CD. 2006. Molecular sensors for optimizing production of alkaloid molecules in Saccharomyces
   cerevisiae. Metabolic Engineering VI, NH Leeuwenhorst, Noordwijkerhout, Netherlands. (Invited
   Speaker)
Smolke CD. 2006. Engineering new molecules for studying and programming cellular systems. Japan-
   America Frontiers of Engineering Symposium, Tsukuba, Japan. (Invited Speaker)
Smolke CD. 2006. Programmable nucleic acid sensors as general tools for optimizing flux through
   synthetic metabolic networks. SIM Annual Meeting, Baltimore, MD. (Invited Speaker)
Smolke CD. 2006. Engineering new molecules for programming complex cellular systems.
   Computational Systems Bioinformatics Conference, Stanford, CA.
Smolke CD. 2006. Engineering new molecules for programming complex cellular systems. International
   Workshop on Logic and Synthesis, Vail, CO. (Invited Speaker)


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                                                                         CD Smolke, Curriculum Vitae


Smolke CD. 2006. Engineering new molecules for studying and programming cellular systems.
   Northwestern University Chemical and Biological Engineering Departmental Seminar, Evanston, IL.
   (Invited Speaker)
Smolke CD. 2006. Programmable molecular switches and sensors: applications in metabolic engineering
   and intelligent therapeutics. Synthetic Biology 2.0, Berkeley, CA. (Invited Speaker)
Smolke CD. 2006. Engineering new molecules for programming cellular systems. Design Principles for
   Biological Systems, Banbury Center Meeting, Cold Springs Harbor Laboratory, NY. (Invited
   Speaker)
Smolke CD. 2006. Programmable molecular switches and sensors: applications in metabolic engineering
   and intelligent therapeutics. Massachusetts Institute of Technology Chemical Engineering
   Departmental Seminar, Cambridge, MA. (Invited Speaker)
Smolke CD. 2006. Ligand-regulated nucleic acid switches as programmable biosensors. Regenerate
   World Congress on Tissue Engineering and Regenerative Medicine, Philadelphia, PA. (Invited
   Speaker)
Smolke CD. 2006. Ligand-regulated nucleic acid switches as programmable molecular sensors. Workshop
   on Biological Large Scale Integration (BioLSI-2), Pasadena, CA. (Invited Speaker)
Smolke CD. 2006. Engineering new molecules for studying and programming cellular systems. Caltech
   Discovery Day, Pasadena, CA. (Invited Speaker)
Bayer TS, Hawkins KH, Kimura Y, Lee H, Lee J, Win M-N, Smolke CD. 2006. Spatial patterning and
   hardwired memory: engineering biomolecules as cellular input/output devices. Institute of Biological
   Engineering Annual Meeting, Tuscon, AZ. (Invited Speaker)
Smolke CD. 2006. The National Academies Keck Futures Initiative: Genomics and Life Engineering
   Conferences. Board of Directors and Beckman Center Advisory Board Meeting, Irvine, CA. (Invited
   Speaker)
Smolke CD. 2006. Expanding the frontiers of molecular medicine through programmable nucleic acid
   sensors. Caltech Division of Chemistry Visiting Committee Presentation, Pasadena, CA. (Invited
   Speaker)
Smolke CD. 2006. Strategies for programming cellular behavior through molecular sensors and switches.
   Caltech Bioengineering Retreat, Warner Springs, CA. (Invited Speaker)
Smolke CD. 2005. Programmable molecular sensors and switches: applications in intelligent therapeutics,
   biosensors, and metabolic engineering. Rensselaer Polytechnic Institute Chemical and Biological
   Engineering Departmental Seminar, Troy, NY. (Invited Speaker)
Smolke CD. 2005. Programmable molecular sensors and switches: applications in intelligent therapeutics,
   biosensors, and metabolic engineering. Cargill Incorporated Seminar, Minneapolis, MN. (Invited
   Speaker)
Smolke CD. 2005. Smart nucleic acid devices for converting diverse biochemical inputs into programmed
   cell behavior. National Academies Keck Futures Initiative Genomics Conference, Irvine, CA. (Invited
   Participant and Poster)
Smolke CD. 2005. From programmable nucleic acid devices to intelligent cells. National Academy of
   Science Frontiers of Science Symposium, Irvine, CA. (Invited Participant and Poster)
Smolke CD. 2005. Programmable nucleic acid molecules. National Academy of Engineering Frontiers of
   Engineering Symposium, Albany, NY. (Invited Participant)
Smolke CD. 2005. Programmable molecular sensors and switches: applications in metabolic engineering,
   intelligent therapeutics, and biosensors. Lawrence Berkeley National Laboratory Synthetic Biology
   Seminar, Berkeley, CA. (Invited Speaker)
Smolke CD. 2005. Programmable molecular sensors and switches: devices for converting biochemical
   information into biological function. Caltech Kavli Nanoscience Inaugural Symposium, Pasadena,
   CA. (Invited Speaker)




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                                                                          CD Smolke, Curriculum Vitae


Smolke CD. 2005. Programmable molecular sensors and switches: applications in metabolic engineering,
   intelligent therapeutics, and biosensors. Caltech Bioengineering Departmental Seminar, Pasadena,
   CA. (Invited Speaker)
Smolke CD. 2005. Programmable molecular sensors and switches: applications in metabolic engineering,
   intelligent therapeutics, and biosensors. University of Southern California Chemical Engineering
   Departmental Seminar, Los Angeles, CA. (Invited Speaker)
Smolke CD. 2005. Engineering alkaloid biosynthesis in Saccharomyces cerevisiae: the application of
   programmable molecular switches to pathway optimization. SIM Annual Meeting, Chicago, IL.
   (Invited Speaker)
Smolke CD. 2005. Programming dynamic cellular response with engineered molecular sensors. NAKFI
   Life Engineering Symposium, San Francisco, CA. (Invited Speaker)
Smolke CD. 2005. Programmable molecular sensors and switches: applications in `intelligent'
   therapeutics and biosensors. Caltech Alumni College, Pasadena, CA. (Invited Speaker)
Smolke CD. 2005. Programmable molecular sensors and switches: applications in `intelligent'
   therapeutics and biosensors. DARPA: Biological Input-Output Systems Program Meeting, Arlington,
   VA. (Invited Speaker)
Smolke CD. 2005. Programmable molecular sensors and switches: applications in intelligent therapeutics,
   biosensors, and metabolic engineering. Stanford Chemical Engineering Departmental Colloquium,
   Palo Alto, CA. (Invited Speaker)
Smolke CD. 2005. Programming dynamic cellular response with molecular sensors. Caltech Alumni
   Seminar Day, Pasadena, CA. (Invited Speaker)
Smolke CD. 2004. Programmable RNA regulators of gene expression: applications in cellular sensors,
   therapeutics, and metabolic circuits. Caltech Biology Division Retreat, Redondo Beach, CA. (Invited
   Speaker)
Selected Presentations
Bayer TS, Hawkins KH, Kimura Y, Lee H, Lee J, Win M-N, Smolke CD. 2005. Engineering hardwired
   DNA memory. iGEM Jamboree, Cambridge, MA. (Oral Presentation)
Bayer TS, Smolke CD. 2005. Understanding signal processing with engineered nucleic acids.
   International Conference Systems Biology, Cambridge, MA. (Poster)
Bayer TS, Smolke CD. 2005. From smart molecules to intelligent cells. Life Engineering Symposium, San
   Francisco, CA. (Poster)
Smolke CD. 2005. Programmable ligand-controlled riboregulators of gene expression. AIChE Annual
   Meeting, Cincinnati, OH. (Oral Presentation)
Smolke CD. 2005. Digital devices for the quantification and identification of large sets of biomolecules
   using engineered nucleic acid sensors. AIChE Annual Meeting, Cincinnati, OH. (Oral Presentation)
Bayer TS, Smolke CD. 2005. Programmable nucleic acid regulators of gene expression. DNA World
   Workshop, Pasadena, CA. (Poster)
Bayer TS, Lee J, Win M-N, Smolke CD. 2004. Programmable RNA-based cellular sensors. Synthetic
   Biology Jamboree, Cambridge, MA. (Oral Presentation)




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