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Certificate Issued To: Work performed at:
Alkemists Pharmaceuticals
Great Herbal Labs LLC 1260 Logan Ave B3
3501 N Causeway Blvd. # 367 Costa Mesa, CA 92626
714-754-HERB (4372)
Metairie, LA 70001 714-668-9972 (FAX)
E-mail: info@alkemist.com
Web Site: www.alkemist.com
Certificate of Analysis 2 of 3: Hoodia gordonii (336705)
High Performance Thin-Layer Chromatography with Photo-Documentation
1 2
Company Name: Great Herbal Labs LLC
Title: Hoodia gordonii (Masson) Sweet ex Decne
Plant Part: aerial parts
Sample Received: 8/17/2007
Sample Description: ~4 grams in a zip lock bag
Form of Botanical: crude plant powder
Appearance: light tan powder
Source: NA
Lot #: (336705) Lanes 4(5µl), 5(5µl)
Sample #: AU22907GHL
Latin Name: Hoodia gordonii (Masson) Sweet ex Decne
Reference Sample #: Lanes 2(3µl), 3(6µl) (AU29006TA2) (aerial part); Lanes 6(6µl), 7(3µl) (AU21906DBL) (aerial part)
Hoodia gordonii authenticated by macroscopic, microscopic &/or TLC studies according
to the reference source cited below, and matching the voucher specimen (AU20604AP)
held at Alkemists Pharmaceuticals, Costa Mesa, CA.
Examiner: SSS 4580
Sample Prep: 0.25g exactly + 5ml CH3OH sonicated + heated in a dry block incubator @ 65° C ~ 1 hr.
Stationary Phase: Silica gel 60, F254, 10 x 10 cm HPTLC plates
Mobile Phase: ethyl acetate: methanol: water [7.7/1.7/1]
Detection: (1) Vanillin/H2SO4 Reagent 110° C 5 min visible light
(2) Vanillin/H2SO4 Reagent 110° C 5 min UV 365 nm
Reference Std: Lanes 1(3µl) and 8(3µl) Aescin (93H1029, Sigma-Aldrich) ~0.1% in CH3OH
Reference Source: Method Developed by Alkemists Pharmaceuticals
Comments & Conclusions: Yellow line = sample origin @ 10mm, red line = solvent front @ 70mm. Lanes 4, 5 are the test
sample Hoodia gordonii (Masson) Sweet ex Decne (336705). Lanes 2, 3 & 6, 7 are the authenticated reference samples
used for comparison. This test sample, Hoodia gordonii (Masson) Sweet ex Decne (336705), is consistent with the
chromatographic profile of the reference samples of Hoodia gordonii used above. This test sample, Hoodia gordonii
(336705) is characteristic of Hoodia gordonii aerial parts.
Authorized by: Sidney Sudberg, Director, Alkemists Pharmaceuticals Report Date: 8/23/2007
This report applies to the sample investigated and is not necessarily indicative of the quality or condition of apparently identical or similar products. This report is
for the exclusive use of the party who requested the report and not for public dissemination or use by third parties, including for promotional purposes, without
the prior written permission of Alkemists Pharmaceuticals, Inc. This report provides technical results for a specific sample and the report shall not be altered,
modified, supplemented or abstracted in any manner. Any violation of these conditions renders the report and its results void.
© 2007 Alkemists Pharmaceuticals, Inc. All Rights Reserved